How does Illumina bead array work?

How does Illumina bead array work?

How Do Illumina Microarrays Work? As DNA fragments pass over the BeadChip, each probe binds to a complementary sequence in the sample DNA, stopping one base before the locus of interest. Allele specificity is conferred by a single base extension that incorporates one of four labeled nucleotides.

What is a Bead Array?

Bead arrays are created by either impregnating beads with different concentrations of fluorescent dye, or by some type of barcoding technology. The beads are addressable and used to identify specific binding events that occur on their surface.

How does a microarray work?

The principle behind microarrays is that complementary sequences will bind to each other. The unknown DNA molecules are cut into fragments by restriction endonucleases; fluorescent markers are attached to these DNA fragments. Then the target DNA fragments along with complementary sequences bind to the DNA probes.

How does CGH microarray work?

Array CGH detects microscopic and submicroscopic deletions and duplications at targeted areas of the genome, including loci of known microdeletion/microduplication syndromes, subtelomeric regions, and pericentromeric regions. Array CGH will also identify marker chromosomes, some cases of mosaicism, and aneuploidy.

What is Illumina BeadArray technology?

Illumina microarray technology (also known as BeadArray technology) uses silica microbeads. On the surface of each array, or BeadChip, hundreds of thousands to millions of genotypes for a single individual can be assayed at once.

How do Illumina microarrays work?

These tiny silica beads are housed in carefully etched microwells and coated with multiple copies of an oligonucleotide probe targeting a specific locus in the genome. Silica beads that self-assemble in microwells on either of two substrates: fiber optic bundles or planar silica slides. How Do Illumina Microarrays Work?

What is a beadchip and how does it work?

On the surface of each array, or BeadChip, hundreds of thousands to millions of genotypes for a single individual can be assayed at once. These tiny silica beads are housed in carefully etched microwells and coated with multiple copies of an oligonucleotide probe targeting a specific locus in the genome.

Why choose Infinium microarray?

Infinium technology produces exceptional data quality and call rates, as well as consistent reproducibility. We offer a broad range of microarray options based on SNP complexity and sample throughput. Since the launch of our first BeadChip, we have innovated microarray solutions to help researchers advance science.